Inhibitors of the ATP/ADP antiporter suppress stimulation of mitochondrial respiration and H+ permeability by palmitate and anionic detergents

Publisher Website
Google Scholar
Abstract
The action of ATP/ADP‐antiporter inhibitors upon the uncoupling effect of palmitate, detergents and ‘classical’ uncouplers has been studied. The uncoupling effect was estimated by stimulation of succinate oxidation and of H+ permeability of rat liver mitochondria in the presence of oligomycin. It is shown that carboxyatractylate (CAtr) and pyridoxal 5‐phosphate (PLP) suppress the uncoupling induced by palmitate and the anionic detergents SDS and cholate, but do not affect that induced by the cationic detergents CTAB, by the non‐ionic detergent Triton X‐100, as well as by the ‘classical’ uncouplers FCCP and DNP. The results are discussed in terms of a concept assuming that the ATP/ADP‐antiporter facilitates the electrophoretic export of hydrophobic anions from mitochondria.