Involvement of natural killer cells in patients with myelodysplastic syndrome carrying monosomy 7 revealed by the application of fluorescence in situ hybridization to cells collected by means of fluorescence‐activated cell sorting

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Abstract
Monosomy 7 is the most frequent chromosome abnormality among patients with secondary myelodysplastic syndrome (MDS). We used fluorescence in situ hybridization (FISH) and fluorescence-activated cell sorting (FACS) in order to clarify the lineage involvement. Four patients, three with de novo MDS and one with secondary MDS, were enrolled in this study. Monosomy 7 was observed in pluripotent stem cells (CD34+Thy-1+), and in B (CD34+CD19+) and T/natural killer (NK) progenitors (CD34+CD7+). The number of abnormal cells of B (CD19+) and T (CD3+) cells was below the cut-off value, but approximately 60% of the NK cells (CD3-CD56+) contained monosomy 7 in three of the patients.