Studies in vitro on the mechanism of the epithelial/mesenchymal interaction in the early fetal thymus

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Abstract
The effect of the mesenchyme on early thymus development was investigated in vitro by culturing tissue recombinants of the epithelium and mesenchyme derived from the earliest fetal thymus primordium. The thymus mesenchyme promoted the development of the epithelium as assessed by expression of epithelial surface molecules such as major histocompatibility complex (MHC) class I and class II antigens; mesenchymes of other organs were similarly effective. We looked for the culture conditions in which the epithelium could normally express the MHC class II antigen without the mesenchyme and found that the insulin‐like growth factors — I and — II were able to support epithelial development under serum‐free conditions. When we cultured the prospective thymus region of a day 9 embryo which had not initiated lymphoid precursor migration, MHC class II antigen was expressed on the epithelium, indicating that lymphoid precursors are not required for early epithelial differentiation. This system provides a means to dissect the complex tissue interactions during the earliest stages of thymus development.