STUDIES OF HUMAN-IGM ANTI-IGG CRYOGLOBULINS .2. ISOELECTRIC-FOCUSING CHARACTERISTICS OF THE REACTIVE ANTIGEN AND RESIDUAL SERUM IGG POOLS
- 1 January 1979
- journal article
- research article
- Vol. 36 (4) , 707-710
Abstract
The co-isoelectric focusing [co-IEF] characteristics of the antigen-Ig[immunoglobulin]G and residual IgG pools in the serum of patients with mixed IgM:IgG cryoglobulinemia were determined. The antigen IgG purified from 5 IgM cryoglobulins and the residual IgG purified from serum depleted of cryoglobulin were radiolabeled and co-IEF in polyacrylamide gels in order to determine if the IgM antiglobulin reacted with IgG having unique physicochemical properties. Electrofocusing profiles of the IgG from 4 sera showed only minor differences when the antigen-IgG was compared to residual serum IgG. In one instance, the IEF profile of the antigen-IgG pool was markedly restricted and of a different charge dispersion than the residual serum IgG. This correlated with the limited antigenic specificity shown for the autologous IgM antiglobulin.This publication has 6 references indexed in Scilit:
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