Growth of JC virus in adult human brain cell cultures

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Abstract
Adult human brain (AHB) cells infected with JC virus (JCV) developed a cytopathic effect (CPE) beginning 12–14 days after infection. Ultrastructurally, 37–40 nm papova virions were seen in the nuclei of infected cells, and both T and V antigen were demonstrated by indirect immunofluorescence. The hemagglutinating titer of JCV in infected AHB cells was 10–40 times higher than the amount of JCV used to initiate infection. AHB cells are more readily available than primary human fetal brain cells, they can be subcultured 15–25 timesin vitro and they support JCV replication after multiple subcultivations. These properties make the AHB cell line useful for propagating JCV.