Sexing of human sperm by discontinuous Percoll density gradient and its clinical application
- 1 October 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Human Reproduction
- Vol. 2 (7) , 573-575
- https://doi.org/10.1093/oxfordjournals.humrep.a136591
Abstract
A discontinuous Percoll density gradient was employed for the selective isolation of human X-bearing sperm. The fraction rich in X-bearing sperm that sedimented through eight steps of a Percoll density gradient was inseminated into healthy volunteers. Six couples achieved pregnancy and each delivered a female baby without abnormality. For further separation, a Percoll density gradient consisting of 12 steps gave a 94% purification of X-bearing sperm (contamination with Y-bearing sperm: 6.4 ± 1.8%) with a mean recovery of 23.3 ± 6.4% (n = 6).Keywords
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