Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase
- 1 October 1966
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 12 (10) , 701-708
- https://doi.org/10.1093/clinchem/12.10.701
Abstract
The procedure for serum alkaline phosphatase determination employing phenolphthalein monophosphate as substrate was evaluated under routine clinical laboratory conditions. A straight-line response to increasing serum concentration was observed, and the reaction was shown to be Mg++ dependent. The method proved to be extremely simple, and the results obtained correlated well with those given by a standard routine procedure involving determination of the liberated inorganic phosphate.Keywords
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