Killer toxin from Hansenula mrakii selectively inhibits cell wall synthesis in a sensitive yeast

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Abstract
Hansenula mrakii secretes extracellularly a killer toxin which kills sensitive Saccharomyces cerevisiae. In protoplasts of this yeast, the killer toxin selectively inhibited the synthesis of alkali‐insoluble acid‐insoluble polysaccharides consisting mainly of β‐glucan, but did not inhibit either the synthesis of other cell wall polysaccharides, such as mannan, chitin and alkali‐insoluble acid‐soluble polysaccharides, or the synthesis of protein. Consistent with these results, the toxin was inhibitory to the β‐(1,3)‐glucan synthetase activity of a cell‐free extract from sensitive S. cerevisiae.