Cotranslational heme binding to nascent globin chains
- 1 July 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 326 (1-3) , 261-263
- https://doi.org/10.1016/0014-5793(93)81803-8
Abstract
Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain.Keywords
This publication has 12 references indexed in Scilit:
- Protein translocation across mitochondrial membranesBioEssays, 1992
- Nonuniform size distribution of nascent globin peptides, evidence for pause localization sites, and a cotranslational protein-folding modelProtein Journal, 1991
- Chlorophyll regulates accumulation of the plastid-encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability.Proceedings of the National Academy of Sciences, 1990
- [28] Hot tritium bombardment technique for ribosome surface topographyPublished by Elsevier ,1988
- O2 binding properties of the product of the central exon of β-globin geneNature, 1981
- Protein-Ligand InteractionsPublished by Springer Nature ,1979
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- Role of Methionine in the Initiation of Haemoglobin SynthesisNature, 1970
- CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONSThe Journal of cell biology, 1970
- Partial resistance of nascent polypeptide chains to proteolytic digestion due to ribosomal shieldingJournal of Molecular Biology, 1967