Mutagenicity Studies with Volatile Metabolites of Halothane

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Abstract
The mutagenicities of 2 volatile metabolites of halothane, 2-chloro,1,1,1-trifluoroethane (CF3CH2Cl) and 2-chloro-1,1-difluoro-ethylene (CF2CHCl), and a presumed halothane metabolite, 2-bromo-2-chloro-1,1-difluoroethylene (CF2CBrCl), were investigated in the Ames bacterial assay system [using Salmonella typhimurium TA98 and TA100 and rat liver microsomes]. Both gas-phase and liquid culture tests were made. The mutagenicity of CF2CBrCl was also studied in a modified Ames test system using rapidly growing cells in enriched liquid medium. The purity of tested compounds was verified by combined gas chromatography and mass spectrometry. In the standard Ames test, CF3CH2Cl and CF2CBrCl were not mutagenic, and CF2CHCl was only weakly mutagenic. CF2CBrCl was detectable as weakly mutagenic in the modified Ames test using rapidly growing cells. Although these results are reassuring, the effects of long-term occupational exposure of humans to halogenated anesthetics are still not fully known.

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