Identification and Measurement of β-Lactam Antibiotic Residues in Milk: Integration of Screening Kits with Liquid Chromatography
Open Access
- 1 July 1995
- journal article
- Published by Oxford University Press (OUP)
- Vol. 78 (4) , 978-986
- https://doi.org/10.1093/jaoac/78.4.978
Abstract
A procedure for identifying and quantitating violative β-lactams in milk is described. This procedure integrates β-lactam residue detection kits with the multiresidue automated liquid chromatographic (LC) cleanup method developed in our laboratory. Spiked milk was deproteinized, extracted, and subjected to reversed-phase LC using a gradient program that concentrated the β-lactams. Amoxicillin, ampicillin, cephapirin, ceftiofur, cloxacillin, and penicillin G were, thus, separated into 5 fractions that were subsequently tested for activity by using 4 kits. (3-lactams in the positive fractions were quantitated by analytical LC methods developed in our laboratory. The LC cleanup method separated β-lactam antibiotics from each other and from interferences in the matrix and also concentrated the antibiotics, thus increasing the sensitivity of the kits to the β-lactam antibiotics. The procedure facilitated the task of identifying and measuring the β-lactam antibiotics that may be present in milk samples.Keywords
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