Characterisation of a Dictyostelium discoideum DNA fragment coding for a putative tRNAValGUU gene.

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Abstract
A genomic DNA fragment from Dictyostelium discoideum was characterized. This DNA, although 74% d(A + T)‐rich, codes for a putative tRNAValGUU. The tRNAVal gene overlaps at its 5′ half with another RNA polymerase III transcription unit. This RNA polymerase III transcription unit can be folded into a tRNA‐like shape and is comprised of significant amounts of invariant and semi‐invariant nucleotides present in all eukaryotic tRNAs. This unit contains the two promoter blocks defined for RNA polymerase III, which are homologous to recently defined promoter elements to the extent of 76–88% (A block) and 86–93% (B block) respectively [Sharp et al. (1981) Proc. Natl Acad. Sci. USA 78, 6657–6661]. Both of the overlapping class III genes are transcribed in germinal vesicle extracts prepared from Xenopus laevis oocytes as a single transcription unit, resulting in an unusually large product compared to primary transcripts of other tRNA genes. The unit is not transcribed in HeLa extracts but it competes very strongly for transcription factor(s) under the conditions of stable transcription complex formation. Although the whole unit is transcribed, it is believed that only one functional product is formed. Therefore we define the tRNA‐like structure, coded for on this class III transcription unit, as a putative tRNA ‘pseudogene’ meaning that, although it is transcribed by RNA polymerase III, it is not likely to mature to a functional tRNA.