Determination of Ethimizol and Two of Its Metabolites in Serum by High-Pressure Liquid Chromatography

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Abstract
A liquid chromatographic assay was developed for the determination of ethimizol [antiinflammatory, anti-allergic and central respiratory analeptic] and 2 of its metabolites in serum. The anlayzed compounds are preseparated from serum by a micro-column packed with an octadecylsilanized silica gel. Components of the micro-column eluate are analyzed on a silica-gel-packed column by means of a high-performance liquid chromatograph fitted with a photometric detector. At 262 nm the detection limit of the injected amount of ethimizol is about 5 ng. The mass spectra of 2 ethimizol metabolites isolated from rat serum are presented. The suitability of the developed assay for pharmacokinetic studies of ethimizol is demonstrated.

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