Adenosine‐5′‐O‐(2‐thiodiphosphate) is a potent agonist at P2 purinoceptors mediating insulin secretion from perfused rat pancreas

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Abstract
1 The effects of a P2 purinoceptor agonist, adenosine 5′-O-(2-thiodiphosphate) (ADP-β-S) have been studied on insulin secretion and flow rate of the isolated perfused pancreas of the rat. 2 In the presence of a moderately stimulating glucose concentration (8.3 mm), ADP-/7-S (4.95–495 nm) evoked a biphasic insulin response in a concentration-dependent manner. A comparison of relative potency between ADP-β-S and adenosine 5′-triphosphate (ATP) showed that ADP-β-S was 100 times more potent than ATP. On the other hand, in the presence of a non stimulatory glucose concentration (4.2 mm), ADP-β-S (165 nm) did not modify the basal insulin secretion. 3 ADP-β-S, at concentrations effective on insulin secretion and also at higher concentrations (1.65 and 16.5 μm), provoked an increase of the pancreatic flow rate in a concentration-dependent manner. 4 Our results show that ADP-β-S is a potent insulin secretory P2 purinoceptor agonist. As it is resistant to hydrolysis it might be useful in studying the effect of activation of the P2 purinoceptor of β cells on insulin secretion in vivo.