CadC Activates pH-Dependent Expression of the Vibrio vulnificus cadBA Operon at a Distance through Direct Binding to an Upstream Region

Abstract
The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, P cadBA , which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the P cadBA DNA was demonstrated, and a CadC binding site centered at −233.5 was mapped by deletion analyses of P cadBA and confirmed by a DNase I protection assay.