Guanosinetriphosphatase activity dependent on elongation factor Tu and ribosomal protein L7/L12.
Open Access
- 1 July 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (7) , 3192-3195
- https://doi.org/10.1073/pnas.75.7.3192
Abstract
Incubation of electrophoretically pure samples of the Escherichia coli 50S ribosomal protein L7/L12 together with elongation factor Tu leads to the hydrolysis of GTP. Addition of elongation factor Ts stimulates this reaction. Elongation factor G cannot replace elongation factor Tu for the ribosome-free GTPase reaction dependent on L7/L12. The data suggest that elongation factor Tu and the protein L7/L12 interact directly at the ribosomal A site.This publication has 14 references indexed in Scilit:
- Effect of Kirromycin on Elongation Factor Tu. Location of the Catalytic Center for Ribosome . Elongation-Factor-Tu GTPase Activity on the Elongation FactorEuropean Journal of Biochemistry, 1977
- Close proximity of Escherichia coli 50 S subunit proteins L7/L12 and L10 and L11Journal of Molecular Biology, 1976
- The protein neighborhood of ribosome‐bound elongation factor TuFEBS Letters, 1976
- ATPase and GTPase activities associated with the 5-S RNA-protein complex of Escherichia coli ribosomesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Kirromycin, an Inhibitor of Protein Biosynthesis that Acts on Elongation Factor TuProceedings of the National Academy of Sciences, 1974
- The Primary Structure of an Acidic Protein from 50‐S Ribosomes of Escherichia coli which is Involved in GTP Hydrolysis Dependent on Elongation Factors G and TEuropean Journal of Biochemistry, 1973
- Studies on Polypeptide Elongation Factor from E. coliThe Journal of Biochemistry, 1972
- Requirement of an Escherichia coli 50 S Ribosomal Protein Component for Effective Interaction of the Ribosome with T and G Factors and with Guanosine TriphosphateJournal of Biological Chemistry, 1972
- The properties of an E. coli ribosomal protein required for the function of factor GArchives of Biochemistry and Biophysics, 1972
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951