THE ROLE OF T-CELL SETS IN THE REJECTION OF A METHYLCHOLANTHRENE-INDUCED SARCOMA (S1509A) IN SYNGENEIC MICE

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 102  (1) , 20-27
Abstract
The ability of different T cell sets to protect mice against a methylcholanthrene-induced sarcoma, S1509a, was examined. I.v. infusion of lymph node and spleen cells from A/J donors immunized with S1509a into normal A/J recipients retarded s.c. growth of S1509a but did not lead to complete eradication of the tumor during a 9-day period of observation. This protective effect was lost if the transferred cells were treated with anti-Thy 1.2 and complement. The ability of different populations of lymphoid cells to retard tumor growth after inoculation with tumor cells s.c. was examined (Winn assay). Nylon-wool-passed cells from lymph nodes and spleens of tumor immunized animals were treated with anti-Ly 1.2 or with anti-Ly 2.2 antiserums and complement and inoculated with tumor cells in normal A/J mice. Ly 1 cells and unfractionated T cells efficiently suppressed tumor growth; Ly 23 cells had little or no effect. When small numbers of Ly 1 cells were injected along with twice as many Ly 23 cells, the growth of the tumor was also inhibited. Histologic examination of inoculated sites at 24-72 h after local transfer showed a more intense mononuclear infiltrate in animals inoculated with tumor cells and T cells from immunized animals than in animals given injection with tumor cells alone or with tumor cells and T cells from nonimmunized animals. Ly 1 cells are capable of retarding the growth of the sarcoma, presumably by eliciting a delayed hypersensitivity reaction. Ly 23 cells, which can mediate cytotoxicity, had little or no effect on tumor growth.

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