ERYTHROCYTE PRESERVATION. V. RELATIONSHIP BETWEEN CHEMICAL CHANGES AND VIABILITY OF STORED BLOOD TREATED WITH ADENOSINE 1
Open Access
- 1 October 1955
- journal article
- research article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 34 (10) , 1509-1512
- https://doi.org/10.1172/jci103202
Abstract
Reversal of storage lesion with adenosine was demonstrated in rabbit and human blood stored at 4[degree]C in ACD and was characterized by in vitro changes and by the effect on post-transfusion survival. Incubation of 3-week stored blood with adenosine for 1 hour at 37[degree]C resulted in the regeneration of organic phosphates, improvement in osmotic fragility, and increase in intracellular K. Changes were sustained for a subsequent 3-week interval at refrigeration temperature. There was an associated improvement in post-transfusion viability of adenosine-treated cells over the second 3-week period as compared with control ACD blood. Evidence seems to indicate that diminished energy potential of the cell is a primary factor in deterioration of the erythrocyte under these conditions of storage.Keywords
This publication has 2 references indexed in Scilit:
- ERYTHROCYTE PRESERVATION .4. INVITRO REVERSIBILITY OF THE STORAGE LESION1955
- ERYTHROCYTE PRESERVATION. III. THE REVERSIBILITY OF THE STORAGE LESION 1Journal of Clinical Investigation, 1954