Reporter gene assays and their applications to bioassays of natural products
- 1 May 2003
- journal article
- review article
- Published by Wiley in Phytotherapy Research
- Vol. 17 (5) , 439-448
- https://doi.org/10.1002/ptr.1312
Abstract
Medicinal plants represent precious resources from which bioactive compounds can be isolated and developed into invaluable therapeutic agents. With the advent of modern drug discovery technologies such as combinatorial chemistry and high‐throughput drug screening platforms, there is an increasing interest in utilizing medicinal plants as a source of drug leads. A wide spectrum of bioassays can be employed for the detection of bioactivity in extracts, fractions, as well as purified compounds of herbal origin. Amongst the different types of bioassays, reporter gene assays are highly versatile and reliable. The present review provides an overview of the most popular reporter genes in terms of their basic methodology, capacities and limitations. The different types of intracellular and extracellular reporter gene products and their potential applications in bioassays of natural products are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.Keywords
This publication has 62 references indexed in Scilit:
- Pseudolaric Acid Analogs as a New Class of Peroxisome Proliferator-Activated Receptor AgonistsPlanta Medica, 2002
- Reporter-gene systems for the study of G-protein-coupled receptorsCurrent Opinion in Pharmacology, 2001
- Current progress in isolation and characterization of toxins isolated from Pfiesteria piscicida.Environmental Health Perspectives, 2001
- Taxol selectively blocks microtubule dependent NF-κB activation by phorbol ester via inhibition of IκBα phosphorylation and degradationOncogene, 1999
- Phytoestrogen intake and prostate cancer: A case‐control study using a new databaseNutrition and Cancer, 1999
- c-Jun/AP-1, but Not NF-κB, Is a Mediator for Oxidant-Initiated Apoptosis in Glomerular Mesangial CellsBiochemical and Biophysical Research Communications, 1997
- Cells lacking CIP1/WAF1 genes exhibit preferential sensitivity to cisplatin and nitrogen mustardOncogene, 1997
- Tetrandrine Inhibits Signal-Induced NF-κB Activation in Rat Alveolar MacrophagesBiochemical and Biophysical Research Communications, 1997
- Use of a luciferase reporter system for characterizing G-protein-linked receptorsCurrent Opinion in Biotechnology, 1995
- Genetic toxicology of flavonoids: the role of metabolic conditions in the induction of reverse mutation, SOS functions and sisterchromatid exchangesMutagenesis, 1986