Temperature Sensitivity of a Sindbis Virus Mutant Isolated from Persistently Infected Aedes aegypti Cell Culture

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Abstract
SV-S, a cloned small plaque mutant of Sindbis virus derived from persistently infected Aedes aegypti cell cultures, was shown also to be an RNA+ temperature-sensitive mutant. It interfered with the replication of SV-W at the nonpermissive temperature as well as the permissive temperature, but did not depress net viral RNA synthesis in cells coinfected with SV-W.

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