Tightly bound pyrophosphate inEscherichia coliinorganic pyrophosphatase
- 26 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (2) , 194-196
- https://doi.org/10.1016/0014-5793(90)80187-n
Abstract
Hexameric inorganic pyrophosphatase of Escherichia coli contains about 1 of ‘structural’ pyrophosphate, which survives gel nitration and prolonged incubation with Mg2+, does not exchange with medium phosphate and pyrophosphate but is removed with 0.8 M perchloric acid. The site of pyrophosphate binding seems to be another than the active site. An additional 0.9 mol of enzyme-bound pyrophosphate is formed in the presence of phosphate and Mg2+ but this pyrophosphate is in fast equilibrium with medium phosphate and appears to be bound to the active site.
Keywords
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