PROPERTIES OF CELL LINES ESTABLISHED FROM TRANSITIONAL CELL CANCERS OF HUMAN URINARY-TRACT

  • 1 January 1977
    • journal article
    • research article
    • Vol. 37  (5) , 1279-1289
Abstract
Characterization studies were done in 8 cell lines (253J, 192B, 639V, 647V, 486P, 575A, 743E and 751G) established from transitional cell cancers of the human urinary tract. Although subtle morphological differences exist among individual lines, each has an epithelial morphology and exhibits multilayering. The doubling times for the cells range from 20-56 h, and at least a 1-3 split can be achieved when they are subcultured every 4th day. Karyotypic analysis revealed a hyperdiploid stemline for each cell line, and the presence of a Y chromosome was confirmed by Q banding in 5 lines. The tumorigenic nature of the cell lines was demonstrated by their production of tumors in hamsters and confirmed by colony formation in agar. The transitional cell cancer lines were free of Mycoplasma, and their glucose-6-phosphate dehydrogenase mobility patterns and karyotypes proved that they were not HeLa [cervical cancer] cells.

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