Direction and Speed of Actin Filaments Moving along Thick Filaments Isolated from Molluscan Smooth Muscle
- 1 September 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 108 (3) , 341-343
- https://doi.org/10.1093/oxfordjournals.jbchem.a123203
Abstract
The active movement of fluorescence-labeled actin filaments along thick filaments isolated from molluscan smooth muscle was observed. Along a single thick filament, actin filaments moved toward the center of the thick filament at the speed of 1.19±0.38 μm 8− (mean±SD, n=42) and detached themselves from it upon reaching the central zone. Movement of actin also occurred in the opposite direction, i.e., away from the center, albeit at a much lower velocity (0.09±0.07 μms−1 n = 17). Thus, the thick filament shows functional bipolarity in terms of velocity but does not determine the direction of the movement.Keywords
This publication has 8 references indexed in Scilit:
- Mg-ATPase activity and motility of native thick filaments isolated from the anterior byssus retractor muscle ofMytilus edulisJournal of Muscle Research and Cell Motility, 1989
- Fluorescent actin filaments move on myosin fixed to a glass surface.Proceedings of the National Academy of Sciences, 1986
- Structure of the myosin projections on native thick filaments from vertebrate skeletal muscleJournal of Molecular Biology, 1984
- Active sliding movement of latex beads coated with skeletal muscle myosin onChara actin bundlesProtoplasma, 1984
- Movement of myosin-coated fluorescent beads on actin cables in vitroNature, 1983
- Matching molecules in the catch mechanism.Proceedings of the National Academy of Sciences, 1982
- The variation in isometric tension with sarcomere length in vertebrate muscle fibresThe Journal of Physiology, 1966
- Electron microscope studies on the structure of natural and synthetic protein filaments from striated muscleJournal of Molecular Biology, 1963