Incorporation of D-(3H)glucosamine into normal and psoriatic epidermal glycoconjugates

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Abstract
Samples of normal, uninvolved and involved psoriatic skin were maintained for 18 h in organ culture in the presence of D-(6-3H)glucosamine, a precursor cell-surface associated carbohydrates in the epidermis. The total incorporation into involved psoriatic epidermis (solublized with 8 mol/l urea, 5% sodium dodecyl sulfate, 10 mmol 2-mercaptoethanol) was less than into normal or uninvolved epidermis. This decrease was found whether specific radioactivites were expressed in terms of area, wet weight or protein content of the epidermis. Electrophoresis revealed that the major labeled component in normal and uninvolved epidermis had a high MW. The labeling of this material was significantly reduced in involved psoriatic epidermis. Using pig epidermis, this material represented mainly extracellular glycosaminoglycans and proteoglycans. The decreased labeling (and presumably synthesis) of these extracellular carbohydrates may be related to the failure of mechanisms controlling cell proliferation and to the altered cell interactions which were found in psoriatic epidermis. Electrophoresis showed that a non-glycosylated protein, MW .apprx. 50,000, present as a single band in normal and uninvolved epidermis, was always present as a doublet band in the involved psoriatic epidermis.

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