Electrogenerated chemiluminescent determination of pyruvate using tris(2,2′-bipyridine)ruthenium(II)
- 1 January 1995
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 120 (10) , 2543-2547
- https://doi.org/10.1039/an9952002543
Abstract
A novel method for the sensitive and selective determination of pyruvate is presented, using an electrogenerated chemiluminescence (ECL) reaction of tris(2,2′-bipyridine)ruthenium(II) in the presence of cerium(III) nitrate, increasing the number and diversity of analytes currently detected by this ECL reaction. Various experimental conditions have been optimized, leading to a calibration over three-orders of magnitude of concentration, with a detection limit of 3.1 × 10–7 mol l–1(27 ppb). Simple methods have also been developed to suppress the interference effects of various compounds that are expected to be present in potential samples from fermentation media.Keywords
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