Detection of IgE antibody to a radiocontrast medium

Publisher Website
Google Scholar
Abstract
Background Very little is known about the mechanistns underlying adverse reactions to radiocontrast medium. On the basis of the clinical features of the adverse reactions, il has generally been considered that an IgE‐dependent mechanism is not involved in these adverse reactions, and only a few studies have demonstrated the presence of IgE antibody to radiocontrast medium iti patient sera. Methods We assayed for IgE antibody to ioxaglie acid (Hexabrix®), a representative radiocontrast medium, in the sera of patients who had developed adverse reactions to ioxaglie acid. A conjugate was prepared by coupling succinyl ioxaglie acid to human serum albumin. Enzyme immunoassay and radioimmunoassay for the detection of IgE antibody to ioxaglie acid in patient sera were constructed by physical adsorption or by eovalent eoupling of the conjugate on solid supports. Results When the radioactivity or the absorbance exceeding the mean plus 3 SD for normal healthy subjects was regarded as positive, IgE antibody was detected in the sera of 47.1% of the patients who had a past history of adverse reactions to ioxaglie acid and 16.2% of those who had experienced adverse reactions to ioxaglie acid within 24 h before the blood collection, although the IgE antibody levels were low. On the other hand, IgE antibody to ioxaglie acid was not detected in the sera of patients with no history of adverse reaetions to ioxaglie acid. Inhibition experiments revealed the presenee of IgE antibody speeific to ioxaglie acid in the serum defined as positive for IgE antibody to ioxaglie acid. The presenee of IgE antibody to ioxaghe acid did not always eorrelate with the activation of mast eells due t o the occurrence of adverse reaelions to ioxaglie acid. Conclusions A small amount of IgE antibody to ioxaglie acid was detected n i the sera of some patients with a history of adverse reactions to ioxaglie acid, and these adverse reactions may be partly explained by the presence of IgE antibody in the serum of at least some patients.