Solution structure of the PX domain, a target of the SH3 domain
- 1 June 2001
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 8 (6) , 526-530
- https://doi.org/10.1038/88591
Abstract
The phox homology (PX) domain is a novel protein module containing a conserved proline-rich motif, We have shown that the PX domain isolated from the human p47(phox) protein, a soluble subunit of phagocyte NADPH oxidase, binds specifically to the C-terminal SH3 domain derived from the same protein. The solution structure of p47 PX has an alpha + beta structure with a novel folding moth topology and reveals that the proline-rich motif is presented on the molecular surface for easy recognition by the SH3 domain. The proline-rich moth of p47 PX in the free state adopts a distorted left-handed polyproline type II helix conformation.Keywords
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