Immunological Characterization of Maize Starch Branching Enzymes
- 1 July 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 72 (3) , 813-816
- https://doi.org/10.1104/pp.72.3.813
Abstract
Highly purified fractions of 3 starch branching enzymes from developing maize (Z. mays L.) endosperm were used to prepare antisera in rabbits. In double diffusion experiments, no immunoprecipitate was observed when branching enzyme IIa or IIb was tested against branching enzyme I antiserum. No immunoprecipitate was formed when branching enzyme I was tested against branching enzyme IIa or IIb antiserum. Increasing amounts of antisera in the above combinations also failed to inhibit enzyme activity. Branching enzyme IIa antiserum cross-reacted and formed spurs with branching enzyme IIb when compared with branching enzyme IIa antigen. Comparison of branching enzyme IIb antiserum with branching enzyme IIa also resulted in an immunoprecipitate. Increasing levels of branching enzyme IIa antiserum inhibited branching enzyme IIb as did the reciprocal combination. Branching enzymes IIa and IIb are immunologically similar while branching enzyme I is distinct. The data support the classification of starch branching enzymes based on genetic, kinetic and chromatographic properties.This publication has 9 references indexed in Scilit:
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