Studies on the flash photolysis of visual pigments. 1. Pigments present in frog-rhodopsin solutions after flash-irradiation

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Abstract
Alkaline digitonin solutions of frog rhodopsin were exposed to an intense 4 msec flash from an 400J xenon discharge tube. A thermally stable phososensitive residue of rhodopsin and a new pigment, P486 ([tau]max = 486 m[mu]) was found after the flash solution was kept in the dark 1 hr. The amount and content of residue was dependent upon flash intensity but independent of addition of 0.01[image] hydroxy-lamine. The residue yield increased inversely with temperature.