A Liver Biopsy Technique for Electrophoretic Evaluation of Largemouth Bass

Abstract
Sixty-four brood size largemouth bass were live biopsied by incision below the pectoral fin and excision of liver tissue to be used in electrophoretic evaluation of individual genetic status. Fifty-four of these fish subsequently were stocked into six brood ponds for production of known intrasubspecific and intersubspecific genetic crosses. Spawning occurred in each pond, indicating minimal effect of biopsy on spawning behavior and physiology. Survival was 81% at the termination of the experiment. All incisions were completely healed and a subsample of biopsied fish revealed no gross or histologic tissue abnormalities.
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