Preparation of a partially desialylated human chorionic gonadotrophin (hCG) and its use for induction of ovulation after ovarian stimulation with human menopausal gonadotrophin

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Abstract
A method for the large scale preparation of partially desialylated human chorionic gonadotropin [hCG] suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation was active in vitro (seminal vesicle weight and ovarian ascorbic acid depletion tests) and its half-life was 13 min in the rat and 75 min in humans. This desialo hCG was effective in inducing ovulation in amenorrheic women. Among 39 induced cycles, 31 ovulations and 5 pregnancies occurred.