Magnesium Metabolism: Basic Aspects and Implications of Ciclosporine Toxicity in Rats

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Abstract
In rapidly growing male Sprague-Dawley rats with an initial body weight of 100 ± 10 g, we investigated how alimentary magnesium (Mg) supply, Mg metabolism and ciclosporine (Ci)-associated nephrotoxicity are interrelated. Food with 100 ppm Mg (lMg) or 1,000 ppm Mg (stMg) or 10,000 ppm Mg (rMg), Ci 20 mg/kg body weight daily or olive oil were applied for 3 months (n = 10/group). Mg concentrations in various compartments were measured by atomic absorption spectrophotometry. Creatinine clearance (Jaffé), urinary N-acetyl-β-D-glucosaminidase (NAG) activity (fiuorometrically), urinary sodium excretion (flame photometry) and osmolality were measured. Histomorphological examination was done and renal renin expression was studied by monoclonal antibodies. Ci reduced the Mg concentration of the femur under lMg(72.6 ± 9.7 vs. 112.6 ± 14.3 mmol/kg dry substance, p

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