Influence of carcinogens and group-specific compounds on DNAse II

1 October 1969

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 47 (10) , 987-989
https://doi.org/10.1139/o69-156

Abstract

A number of cancer-causing and group-specific compounds were tested for their effects on the activity of DNAse II. The following were almost completely inhibitory: iodoacetic acid, N-bromosuccinimide (at an N-bromosuccinimide/enzyme level ≥ 24), and H₂O₂ (at an H₂O₂/enzyme level > 10 000). Either noninhibitory or less than 30% inhibitory were iodoacetamide and diisopropylfluorophosphate. Noninhibitory were such carcinogens as beta-butyrolactone, diepoxybutane, 3-hydroxyxanthine, and ascaridole. Also noninhibitory was malonaldehyde.From these results (and others in the literature), it was concluded that (1) the carcinogens tested (at least in their unmetabolized forms) might not act directly on DNAse II in the critical reaction of the carcinogenic process, and (2) tryptophan, methionine, and/or histidine residues play important roles in the enzyme activity.

Keywords

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