Quantitative Real-Time PCR Assay for Detection of Ehrlichia chaffeensis
Open Access
- 1 August 2003
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (8) , 3870-3872
- https://doi.org/10.1128/jcm.41.8.3870-3872.2003
Abstract
A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis . The assay is species specific and provides quantitative results in the range 10 to 10 10 gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis .Keywords
This publication has 21 references indexed in Scilit:
- Characterization of the rRNA Genes ofEhrlichia chaffeensisandAnaplasma phagocytophilaDNA and Cell Biology, 2002
- Antibodies Highly Effective in SCID Mice During Infection by the Intracellular BacteriumEhrlichia chaffeensisAre of Picomolar Affinity and Exhibit Preferential Epitope and Isotype UtilizationThe Journal of Immunology, 2002
- Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila.International Journal of Systematic and Evolutionary Microbiology, 2001
- The Human Ehrlichioses in the United StatesEmerging Infectious Diseases, 1999
- Ehrlichia ewingii,a Newly Recognized Agent of Human EhrlichiosisNew England Journal of Medicine, 1999
- Natural history of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) in the piedmont physiographic province of Georgia.Journal of Parasitology, 1997
- Experimental Transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) Among White-Tailed Deer by Amblyomma americanum (Acari: Ixodidae)Journal of Medical Entomology, 1995
- Ehrlichia muris sp. nov., Identified on the Basis of 16S rRNA Base Sequences and Serological, Morphological, and Biological CharacteristicsInternational Journal of Systematic and Evolutionary Microbiology, 1995
- Serologic Diagnosis of Human Ehrlichiosis Using Two Ehrlichia canis IsolatesThe Journal of Infectious Diseases, 1991
- Substrate utilization by Ehrlichia sennetsu and Ehrlichia risticii separated from host constituents by renografin gradient centrifugationJournal of Bacteriology, 1988