Generation of Thiocillin Variants by Prepeptide Gene Replacement and in Vivo Processing by Bacillus cereus
- 13 November 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (48) , 17563-17565
- https://doi.org/10.1021/ja908777t
Abstract
The thiocillins are natural-product antibiotics derived from ribosomally encoded peptides that undergo extensive posttranslational modifications to yield the mature trithiazolylpyridine-containing macrocyclic compound. Poor pharmacokinetic properties have prevented the clinical use of these highly potent antibiotics. Through in vivo manipulation of the gene responsible for production of the thiocillin precursor peptide, we have generated 65 novel thiocillin variants, allowing us to explore structure−activity relationships involved in both precursor peptide maturation and antibiotic activity.This publication has 24 references indexed in Scilit:
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