Purification of Rat Angiotensinogen
- 1 March 1986
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 16 (1) , 45-59
- https://doi.org/10.1080/00327488608062458
Abstract
A method of purifying rat angiotensinogen in three chromatography steps with a yield 3–4 times better than previous methods is described. Using chromatofocusing media for two steps and DEAE-affigel blue for the third step it was possible to separate angiotensinogen into three major peaks with pI of 5.25 (peak B), 4.80 (peak C) and 4.50 (peak D). Peaks B and C were completely purified with recoveries of 12% and 17% and specific activities of 21.8 and 20.0 μ Al/mg protein respectively. Analytical SDS-PAGE showed a 53, 000 dalton band in both peaks with additional 51, 000 and 57, 000 dalton bands in peak C.Keywords
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