Rapid Determination of Acyclovir in Plasma by Reversed Phase High-Performance Liquid Chromatography

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Abstract
A high performance liquid chromatographic method has been developed for the determination of a new antiviral drug : Acyclovir [9-(2 hydroxyethoxymethyl) guanine], in human plasma, using a reversed-phase method, with ultraviolet detection at 254 nm. The internal Standar acetaminophen, was used as an aid to quantitation. The plasma sample was deproteinized supernatant was cleanup with methylene chloride, then directly analyzed in the chromatographic system. The limit of detection was about 0.25 μg/ml cf plasma with a precision of ± 7.4 %. The method is used for quantitative acyclovir in plasma from renal failure patients, with satisfactory accuracy and precision. Endogenous substanes and a variety of drugs concomitanly used in antiviral therapy did not interfere with the assay.

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