Chromatographic Resolution of Amino Acids Using Tartaric Acid Mono-N-octylamide as Mobile Phase Additive
- 1 February 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (2-3) , 551-571
- https://doi.org/10.1080/01483918608076653
Abstract
Chromatographic resolution of enantiomeric amino acids by a chiral ligand exchange system is presented using a novel chiral mobile phase additive, (R,R)-tartaric acid mono-n-octylamide (TAMOA) which sufficiently complexes copper II or nickel II ions. The chiral system is generated by dynamically coating a reversed phase packing, and shows selectivity for many amino acids. Under certain conditions also norepinephrine can be partially resolved. Retention and enantion separation mechanisms in conjunction with the mobile phase composition and the ratio of TAMOA to the metal ion are discussed.Keywords
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