Trypanosoma brucei: a surface antigen mRNA is discontinuously transcribed from two distinct chromosomes.
Open Access
- 1 April 1985
- journal article
- research article
- Published by Wiley in The EMBO Journal
- Vol. 4 (4) , 995-998
- https://doi.org/10.1002/j.1460-2075.1985.tb03729.x
Abstract
The mRNAs for variant surface glycoproteins (VSGs) and many other proteins in Trypanosoma brucei start with the same sequence of 35 nucleotides, encoded by a separate mini‐exon. There are approximately 200 mini‐exon genes per trypanosome and these are highly clustered on large chromosomes. We have found two trypanosome variants that express a VSG gene located on a small, 225‐kb chromosome. Each gene yields a mRNA containing the 35‐nucleotide sequence even though the 225‐kb chromosome does not contain a complete mini‐exon gene. These results provide a strong support for the hypothesis that transcription of protein‐coding genes in trypanosomes is discontinuous.This publication has 40 references indexed in Scilit:
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