Gas-liquid Chromatographic Determination of Optical Purity of Allethrolone and Allethronyl Chrysanthemate

Abstract
Gas-liquid chromatographic(GLC) method was developed for the determination of the optical purity of allethrolone and allethrolone moiety in allethronyl chrysanthemate. Allethrolone was reacted with N-trifluoroacetyl(TFA)-l-prolyl chloride to give diastereoisomer esters, which were separated from each other on a 10% silicone DC QF-1 column (3 mm i.d.×2.25 m) and the optical isomers of allethrolone were determined by their peak areas. (+)-Allethronyl (+)-trans-chrysanthemate was hydrolyzed with 0.5 n methanolic potassium hydroxide and the liberated allethrolone was extracted from the hydrolyzate with chloroform and its isomer ratio was determined.

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