Glutathione, oxidized and reduced, in some dipterans treated with 1,1,1-trichloro-2,2-di-(p-chlorophenyl)ethane

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Abstract
In the housefly, Musca domestica, the content of reduced plus oxidized glutathione is about 2 [mu]equiv./g of tissue, with a GSH/GSSG ratio approximating to 7:1. The same values hold for the mosquito, Anopheles albimanus. Insignificant differences were observed in the GSH/GSSG ratio between DDT-treated or untreated animals or between DDT-susceptible and DDT-resistant strains of the two species. Minor differences in total GSH content between strains may be due to factors other than resistance. No differences were observed in the NADPH-glutathione-reductase activities of resistant and susceptible strains of houseflies: an average rate of NADPH oxidation of approximately 3 [mu]moles/g hr. was obtained in both cases. Paper chromatography of fly extracts showed GSH to constitute the principal free thiol to be present in the tissues.