Single fluorescent protein-based Ca2+sensors with increased dynamic range
Open Access
- 29 June 2007
- journal article
- Published by Springer Nature in BMC Biotechnology
- Vol. 7 (1) , 37
- https://doi.org/10.1186/1472-6750-7-37
Abstract
Genetically encoded sensors developed on the basis of green fluorescent protein (GFP)-like proteins are becoming more and more popular instruments for monitoring cellular analytes and enzyme activities in living cells and transgenic organisms. In particular, a number of Ca2+ sensors have been developed, either based on FRET (Fluorescence Resonance Energy Transfer) changes between two GFP-mutants or on the change in fluorescence intensity of a single circularly permuted fluorescent protein (cpFP).Keywords
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