ASSOCIATION OF PHENOTYPIC REVERSION OF TRANSFORMED-CELLS INDUCED BY INTERFERON WITH MORPHOLOGICAL AND BIOCHEMICAL-CHANGES IN THE CYTOSKELETON

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (10) , 4174-4184
Abstract
The distribution of microfilament bundles, intermediate filaments, microtubules, vinculin plaques and extracellular fibronectin matrices was examined by indirect immunofluorescence in X-ray-transformed C3H/10T1/2 [mouse fibroblast] cells. The transformed phenotype correlated with a lack of actin cables and vinculin-containing plaques. Prolonged treatment of these transformed cells with interferon resulted in a reversion of the transformed phenotype which was associated with the reappearance of extensive microfilament bundles together with vinculin-containing plaques. Both interferon-treated and untreated transformed cells displayed a similar distribution of mitochondria, intermediate filaments, microtubules, and fibronectin. Significant qualitative and quantitative differences between the 2 types of cells were found in the proteins associated with the cytoskeletal framework by 2-dimensional gel analysis.

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