Isolation of a fourth cysteinyl‐containing peptide of the α‐subunit of the F1 ATPase from Escherichia coli necessitates revision of the DNA sequence
- 3 March 1986
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 197 (1-2) , 121-124
- https://doi.org/10.1016/0014-5793(86)80310-6
Abstract
The rapid determination of cysteinyl residues by Creighton's method [(1980) Nature 284, 487‐489] led to the discovery of a discrepancy between protein and DNA sequence data in the α‐subunit of the F1 ATPase from Escherichia coli [(1984) Arch. Biochem. Biophys. 229, 320‐328]. We have isolated a cysteinyl‐containing decapeptide from the α‐subunit with a protein sequence (AGCAMGEYFR) which is only partially recognizable from DNA data. Re‐sequencing of DNA in the region coding for the peptide has resulted in two corrections: insertion of a cytosine before position 715 and deletion of a thymine at position 731 of the uncA gene.Keywords
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