Purification and Properties of Choriogonadotropin from Human Term Placenta

Abstract
Affinity chromatography was applied successfully to the isolation of human choriogonadotropin from human term placenta. In a single step, a 57-fold purification was achieved. Evidence is presented which indicates that the product contains intact hormone as well as its free .alpha.-subunit. Preliminary data suggest that a variant form of .alpha.-subunit is also present which has serine at its N-terminal and contains relatively little arginine and proline compared to the authentic .alpha.-subunit.

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