Chloroquine Removal of HLA Antigens from Platelets for the Platelet Immunofluorescence Test
- 5 March 1985
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 48 (3) , 156-159
- https://doi.org/10.1111/j.1423-0410.1985.tb00163.x
Abstract
Chloroquine-treated [human] platelets did not react with HLA antisera in a platelet suspension immunofluorescence test (PSIFT), while the reactions with platelet-specific antibodies (P1A1, Baka) seemed even stronger after treatment. The method might be useful in discerning HLA and platelet-specific antibodies without absorption procedures. The treatment causes death of a proportion of the cells, with nonspecific fluorescence making the reading difficult. This may be circumvented by using special phase-contrast lenses.This publication has 9 references indexed in Scilit:
- Leka, a New Platelet Antigen Absent in Glanzmann's ThrombastheniaVox Sanguinis, 1984
- HLA antigens and maternal antibodies in allo‐immune neonatal thrombocytopeniaTissue Antigens, 1983
- Chloroquine dissociation of antigen‐antibody complexesTransfusion, 1982
- Ability of thrombocytes to acquire HLA specificity from plasmaBlood, 1982
- Post‐transfusion Purpura: a Serological and Immunochemical StudyBritish Journal of Haematology, 1981
- Neonatal alloimmune thrombocytopenia: detection and characterization of the responsible antibodies by the platelet immunofluorescence testBlood, 1981
- Absence of platelet-specific alloantigens in Glanzmann's thrombastheniaBlood, 1981
- Post-transfusion thrombocytopenic purpura: Immunological and clinical studies in two cases and review of the literatureAnnals of Hematology, 1980
- A Simple Immunofluorescence Test for the Detection of Platelet AntibodiesBritish Journal of Haematology, 1978