COMMON LEUKEMIA-ASSOCIATED ANTIGEN OF DBA-2 MOUSE LEUKEMIA DETECTED BY TUMOR REJECTION AND COMPLEMENT-DEPENDENT CYTO-TOXICITY ASSAYS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 70  (6) , 769-776
Abstract
A cytotoxic antibody for L1210 leukemia cells was found in the (C57BL/6 .times. DBA/2)F1 (BDF1) mice immunized with L1210 leukemia cells infected with ts [temperature sensitive] mutant of HVJ [hemagglutinating virus of Japan] (HVJ-pi) and challenged several times with uninfected L1210 leukemia cells. These immune mice fell into 2 categories; high and low responders regarding the titer of cytotoxic antibody produced. The antigen defined by this cytotoxic antibody was present on leukemia cells originating in DBA/2 mice but not on leukemia induced by passage-A Gross virus or spontaneous mammary tumors. This serological cross-reactivity among L1210, P388 and L5178Y leukemia cells was substantially confirmed by the observation of cross protection against challenge with DBA/2 leukemia cells in immune BDF1 mice. These findings strongly suggested the presence of a common DBA/2 leukemia-associated antigen different from known cell-surface antigens of murine leukemia. Non-cytopathic, viable HVJ-pi-infected tumor cells are very effective as an immunogen for inducing tumor immunity.

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