Isolierung von Riboflavin-Mangelmutanten von Saccharomyces cerevisiae

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Abstract
Riboflavin deficient mutants of S. cerevisiae were isolated using a yeast extract peptone-dextrose medium supplemented with 10 [mu]g riboflavin/ml. After treatment with ethyl methanesulfonate, 0.4% of the surviving cells or 0.2% of the isolated biochemical mutants were deficient in riboflavin. Seven of the 14 riboflavin deficient mutants were tested for temperature dependence but no such dependence was found. Two mutants accumulated a green fluorescing substance. Cross-feeding tests were not successful.