Evaluation of a Real-Time Nucleic Acid Sequence-Based Amplification Assay Using Molecular Beacons for Detection of Human Immunodeficiency Virus Type 1
- 1 June 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (6) , 2280-2282
- https://doi.org/10.1128/jcm.02187-05
Abstract
We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.Keywords
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