Abstract
Crossed immunoelectrophoresis in various modifications has directly revealed the immunological partial identity between isolated major allergens of timothy pollen (antigens 19 and 25) and the corresponding pollen allergens of false oat, orchard and rye grass, using rabbit antibodies raised against all four grasses. The inhibitory effects of antigens 19 and 25 relative to those of whole pollen extracts have been determined by means of RAST, inhibiting serum pools from selected grass-pollen-allergic patients and using insolubilized whole pollen extracts from the four grasses as allergosorbents. The inhibitory effects of antigens 19 and 25 were determined to be 97, 91, 92 and 73% of those of whole extracts of timothy, false oat, orchard and rye grass, respectively.

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